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American Society of Microbiology ABMM Exam Syllabus Topics:

American Society of Microbiology ABMM Sample Questions:

1. A clinical microbiology laboratory encounters a Gram-negative bacillus isolated from a bloodstream infection. The isolate exhibits resistance to multiple antibiotics, including carbapenems. Further phenotypic testing reveals the production of a metallo-beta-lactamase (MBL). Which of the following antibiotic combinations might still retain activity against this isolate?

A) Ceftazidime-avibactam
B) Aztreonam
C) Piperacillin-tazobactam
D) Aztreonam-avibactam

2. A microbiology laboratory is investigating a possible outbreak of Salmonella infection. Serological testing of patient isolates reveals they all possess the same O antigen but different H antigens. This suggests:

A) Contamination in the laboratory affecting multiple samples.
B) Multiple sources of infection with unrelated Salmonella strains.
C) A common ancestral strain that has undergone flagellar antigen variation.
D) A single point source of infection with a genetically stable strain.

3. A patient with a history of recurrent Clostridioides difficile infection (CDI) is being considered for fecal microbiota transplantation (FMT). Pre-FMT screening of the donor stool sample should include comprehensive testing to exclude potential transmission of enteric pathogens. Which of the following would be the MOST critical to rule out in the donor stool?

A) Quantification of the overall microbial diversity using 16S rRNA gene sequencing.
B) Presence of non-toxigenic C. difficile strains.
C) Evidence of asymptomatic carriage of multidrug-resistant organisms (MDROs) such as vancomycin- resistant enterococci (VRE) or carbapenem-resistant Enterobacterales (CRE).
D) Detection of common antibiotic resistance genes not currently expressed.

4. What is considered the "gold standard" for routine monitoring of the lethality of steam sterilization (autoclave) cycles in healthcare facilities?

A) Use of chemical indicator strips placed inside each sterilized pack.
B) Daily check of autoclave cycle printouts for correct time, temperature, and pressure.
C) Regular use of biological indicators containing spores of Geobacillus stearothermophilus.
D) Performing a Bowie-Dick test at the beginning of each day for pre-vacuum sterilizers.

5. A 55-year-old male with a history of poorly controlled diabetes mellitus develops a necrotizing fasciitis of his left lower extremity following a minor abrasion sustained while gardening. Initialcultures of the wound reveal a polymicrobial flora, including Gram-positive cocci in chains and Gram-negative bacilli. Over the next 48 hours, despite broad-spectrum antibiotic therapy, the patient's condition deteriorates, and new bullae with hemorrhagic fluid appear. Gram stain of this fluid now predominantly shows large, Gram-positive bacilli with subterminal spores. Which of the following virulence factors is MOST likely contributing to the rapid tissue destruction observed in this patient?

A) Protein A
B) Lipopolysaccharide (LPS)
C) Exotoxin B (SpeB)
D) Lecithinase (alpha-toxin)

Solutions: