ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) : ASCP-MLT

  • Exam Code: ASCP-MLT
  • Exam Name: MEDICAL LABORATORY TECHNICIAN - MLT(ASCP)
  • Updated: Oct 13, 2026
  • Q & A: 562 Questions and Answers

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ASCP ASCP-MLT Exam Syllabus Topics:

SectionWeightObjectives
Hematology20-25%- White blood cell morphology and diseases
- Red blood cell physiology and disorders
- Routine and automated hematology procedures
- Hemostasis and coagulation testing
Blood Banking (Immunohematology)15-20%- Blood components and storage
- Compatibility testing
- Antibody detection and identification
- Transfusion reactions and adverse events
- Blood group systems and antigens
Urinalysis and Other Body Fluids5-10%- Urine formation and composition
- Physical, chemical, and microscopic analysis
- Semen and amniotic fluid testing
- Cerebrospinal, serous, and synovial fluids
Clinical Chemistry20-25%- Carbohydrates, lipids, proteins, and enzymes
- Electrolytes, acid-base balance, and blood gases
- Toxicology and liver/kidney function tests
- Endocrinology and therapeutic drug monitoring
Immunology and Serology5-10%- Infectious disease serology
- Immune system principles and components
- Immunologic testing methodologies
- Autoimmune and immunodeficiency disorders
Microbiology15-20%- Identification of pathogenic microorganisms
- Specimen collection and culture techniques
- Mycology, parasitology, and virology
- Bacteriology and antimicrobial susceptibility
Laboratory Operations5-10%- Laboratory mathematics and instrumentation
- Pre-analytical, analytical, and post-analytical processes
- Laboratory safety and regulatory compliance
- Quality control and quality assurance

ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

Question #1

AHG must be added to the cells immediately following washing. Antibodies may elute from the cells if the cells are allowed to sit in saline without the addition of AHG.
Which one of the following may cause a FALSE-NEGATIVE result with antiglobulin techniques?

  • A. Patient's blood specimen was collected in silicone gel tubes
  • B. Red cell/AHG test sample is over-centrifuged
  • C. Addition of AHG is delayed for 40 minutes or more after final saline wash
  • D. Saline used for washing the test sample has been stored in squirt bottles
  • E. Patient's red cells have an antibody coating them
Reveal Solution  Discussion  0

Correct Answer: C  🗳️

Question #2

Hematology
What is Coombs sera comprised of:

  • A. Complement
  • B. Anti-human globulins
  • C. Anti-red cell globulins
  • D. Indicator red cells
Reveal Solution  Discussion  0

Correct Answer: B  🗳️

Question #3

Public health guidelines recommend that manipulation of samples for influenza testing be done inside a safety cabinet. Safety goggles could be worn if the specimen or procedure may be prone to splashes or sprays of infectious material. Utility gloves are not necessary.
Public health guidelines recommend that laboratory specimens for influenza testing must be manipulated using which of the following safety precautions?

  • A. Safety cabinet
  • B. Safety goggles
  • C. Utility gloves
  • D. None of the above
Reveal Solution  Discussion  0

Correct Answer: A  🗳️

Question #4

The difference between plasma and serum is that:

  • A. plasma is obtained from a clotted specimen.
  • B. serum is the cells of unclotted blood.
  • C. serum is obtained from a nonclotted specimen
  • D. plasma is from an unclotted specimen.
Reveal Solution  Discussion  0

Correct Answer: D  🗳️

Question #5

The part of the microscope that holds the objective lenses is called the _______________.

  • A. condenser
  • B. fine adjustment
  • C. revolving nosepiece
  • D. objective
Reveal Solution  Discussion  0

Correct Answer: C  🗳️

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